Apple Colored Birefringence
Many pathologists use Congo red to make a diagnosis of amyloid and state the common opinion that in polarized light, Congo red-stained amyloid shows apple-green birefringence, sometimes called. We studied 160 papers on Congo red-stained amyloid and found that virtually all reported just green birefringence or apple-green birefringence, even though only 31% of the illustrations showed a pure green color. 2 There were discrepancies between the colors reported and illustrated in 66% of the figures, and these were mostly discrepancies.
The idea that green, and only green, is essential for the diagnosis of amyloid has persisted almost universally, and virtually all mentions of Congo red‐stained amyloid say that it just shows "green birefringence" or "apple‐green birefringence.". The Congo Red stain is a vital histological tool widely used in pathology and biomedical research, primarily for detecting amyloid deposits in tissue samples. Known for its unique ability to exhibit apple-green birefringence under polarized light, Congo Red stain plays a crucial role in diagnosing diseases such as amyloidosis and Alzheimer's disease.
5 immunology-csbrp
Beyond amyloid detection, it is also. When polarized light passes through the Congo Red-stained amyloid, the characteristic apple. When stained with Congo red and observed under polarized light, amyloid has a characteristic "apple green" birefringence as seen here in deposits around small arteries and within the cortex of the adrenal gland of a patient with multiple myeloma and excessive light chain production (AL amyloid).
Apple Green Birefringence. Posted by Dr Vijay Shankar S Dec 8, 2016 Diseases of Immune System, General Pathology Polarizing microscopy: It is a type of Optical microscopy which uses polarized light for examination of specimens/slides. This type of microscopy was developed initially to examine crystalline structures found in rocks and.
POLARIZING MICROSCOPY made SIMPLE! Apple Green Birefringence - YouTube
Many pathologists use Congo red to make a diagnosis of amyloid and state the common opinion that in polarized light, Congo red-stained amyloid shows apple-green birefringence, sometimes called apple-green dichroism. Is this opinion correct? A cursory glance at published micrographs said to illustrate this color reveals that most show more than one color and that some do not even show green. In.
Like most people working on amyloid, Colombat et al.1 report that Congo red-stained amyloid shows "green birefringence" or "apple-green birefringence," although their figures (5b, 6b, 7b, and 7d-f) show various colors, and in at least two (7e and 7f), green is difficult to see. We wrote to Kidney International in 2012 to point out a similar discrepancy between so-called "apple. Most people seem convinced that the appropriate description of the properties of Congo red-stained amyloid is 'apple-green (or green) birefringence', although only 20% of images show pure green, 47.
