Ghost Dye Live Dead
Ghost Dye™ Violet 510 is an amine reactive viability dye that can be used to discriminate viable from non. Explore fixable viability dyes for flow cytometry with 14 LIVE/DEAD dye options which allow for cleaner separation and identification of cell populations. Ghost Dyes bind irreversibly to amine groups and are resistant to subsequent washing, fixation, and permeabilization, leaving behind a bright and stable fluorescent signal.
On live cells, this class of dye is only able to bind to surface amines. Dead cells with compromised membranes allow the dye to permeate and bind amine groups of intracellular proteins within the cytoplasm. This results in.
Why use viability dyes in flow cytometry experiments?
This results in a much higher fluorescence (roughly 50x greater) in dead cells, allowing live cells to be easily identified. Fixable viable dyes, aptly named Zombie Dyes™ (Bio-Legend), Ghost Dyes™ (Tonbo Biosciences) etc., are popular due to their ease of use and availability in many colors to suit different panel designs. Flow cytometric analysis of live and fixed/permeabilized human peripheral blood mononuclear cells, combined and stained with Ghost Dye Violet 510 Viability Dye.
Viable gate is indicated. The Ghost Dye 510 is an inexpensive flow cytometry reagent that allows for efficient labeling of dead cells and subsequent multi-color analysis. After appropriate compensation, the 510 dye is a particularly useful color option for complex panels requiring the use of multiple colors off the violet laser.
Ghost Dye™ Violet 510 Fixable Viability Dye | Cell Signaling Technology
Ghost Dye™ Violet 510 from Cytek is an amine-reactive viability dye used in flow cytometry when it will be necessary to fix and/or permeabilize the cells after staining. This reagent has an excitation peak at 413 nm and an emission peak at 508 nm. Examples of theses dyes are the live/dead fixable dyes, the eFluor fixable dyes, the Horizon dyes, the Biolegend Zombie dyes and the Ghost dyes.
Although the dyes can be fixed, this is not mandatory so they can also be used to identify dead cells in sorting experiments. The Live/Dead Ghost dye 780 is a fluorescent dye used to distinguish live and dead cells in flow cytometry. It binds to nucleic acids and emits a far-red fluorescent signal, allowing for the identification and separation of viable and non.
